Abstract
Legumes are a large and economically important family, containing a variety of crop plants. Alongside different cereals, some fruits, and tropical roots, a number of leguminosae evolved for millennia as crops with human society. One of these legumes is Pisum sativum L., the common garden pea. In the past, breeding has been largely selective on improved above-ground organs. However, parameters, such as root-growth, which determines acquisition of nutrients and water, have largely been underestimated. Although the genome of P. sativum is still not fully sequenced, multiple proteomic studies have been published on a variety of physiological aspects in the last years. The presented work focused on the connection between root length and the influence of the microsomal root proteome of four different pea cultivars after five days of germination (cultivar Vroege, Girl from the Rhineland, Kelvedon Wonder, and Blauwschokker). In total, 60 proteins were identified to have significantly differential abundances in the four cultivars. Root growth of five-days old seedlings and their microsomal proteome revealed a similar separation pattern, suggesting that cultivar-specific root growth performance is explained by differential membrane and ribosomal protein levels. Hence, we reveal and discuss several putative root growth protein markers possibly playing a key role for improved primary root growth breeding strategies.
Highlights
Pea cultivation developed together with human society for about 11,000 years [1]
Summarizing, proteomic approaches have the potential to be used as alternative techniques to QTL mapping
Our shotgun proteomics approach enabled distinguishing different pea cultivars according to their root microsomal proteome linked to their root phenotypes
Summary
Pea cultivation developed together with human society for about 11,000 years [1]. Nowadays, Pisum sativum L. is one of the economical important legumes, including Glycine max (L.) MERR.(soybean), Phaseolus L. (bean), Cicer arietinum L. (chickpea), and Arachis hypogaea L. (peanut). Pea cultivation developed together with human society for about 11,000 years [1]. In the most recent trends, pea seedlings are used to produce protein concentrates for protein powders as an alternative to dairy-derived whey proteins or glycine proteins. According to FAOSTAT data world production of the garden pea has more than quadrupled in the last 30 years [2]. The pea has been investigated as a model with respect to several physiological aspects and, interestingly enough, mapping the pea genome has lagged behind other crops because it has such a large and complex genome [3]. Aside from this, databases with information on pea genomic markers, genetic maps, quantitative trait loci (QTLs), Proteomes 2017, 5, 8; doi:10.3390/proteomes5010008 www.mdpi.com/journal/proteomes
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