Proteomic profiling of platelet signalling

Abstract

Platelets play a crucial role in hemostasis; activating and aggregating to arrest bleeding following vascular injury. Platelet activation is a complex and dynamic process, involving the co-ordination of numerous receptors to initiate shape change and aggregation. Under pathological conditions, alterations in the normal platelet response can favor a prothrombotic state and increase the risk of acute coronary syndromes (ACS). Receptor stimulation and the tyrosine phosphorylation of key signaling molecules underpin platelet activation in both hemostasis and cardiovascular disease. A lack of nucleus and low mRNA levels makes protein function the primary focus of platelet research. Advancements in proteomic technologies now allow for comprehensive analysis of the platelet proteome and its associated post-translational modifications. In this review, recent applications of proteomics in platelet signaling studies are discussed with particular focus on the elucidation of novel phosphorylation events following receptor activation.