Proteomic Profiling of High-grade Glioblastoma Using Virtual experimental 2DE

Stanislav N Naryzhny,Maria A Maynskova

doi:10.4172/jpb.1000402

Abstract

Identification and quantitative analysis of different proteoforms (protein species) presented in a cell line generated from high grade glioblastoma was performed using two-dimensional electrophoresis (2DE), mass spectrometry (ESI LC-MS/MS), and immunodetection. A 2DE protein map containing an extensive data set comprising 937 spots with 1542 unique protein identifications (proteoforms) of 600 genes was obtained. Additionally, another set of experiments was performed where 16012 proteoforms coded by 4050 genes were identified by MS/MS according to their position in 96 gel sections (pixels). A special attention has been paid to the proteins that are the potential biomarkers of glioblastoma. The list of these biomarkers was compiled from literature. Next, we generated the graphs with theoretical and experimental information about proteoforms coded by the same gene. Such a virtualexperimental representation allowed better visualization of the state of these gene products. Many proteins, potential biomarkers of glioblastoma as well, are characterized by high numbers of protein species. We assume that these species could be a potential source of highly specific biomarkers of glioblastoma.

Highlights

High grade glioma is the most common brain tumor with a very poor prognosis
We found that proteome profiles in normal and glioblastoma cell lines are very similar but levels of several proteins have prominent differences between norm and cancer [4]
We have performed high resolution two-dimensional electrophoresis (2DE) of proteins extracted from glioblastoma cells followed by mass spectrometry or immunodetection (Western blot)

Summary

Introduction

High grade glioma (glioblastoma multiform, WHO grade IV) is the most common brain tumor with a very poor prognosis. As glioblastoma cells are very heterogeneous, identifying of biological signatures for each subtype through protein biomarker profiling is a high priority. In our previous experiments, searching for protein signatures and biomarkers for gliomas we used cell lines to obtain proteomics information specific to this disease [4].

Results

Conclusion