Abstract
Drought stress is one of the most important abiotic stresses that plants face frequently in nature. Under drought conditions, many morphological, physiological, and molecular aspects of plants are changed and as a result plants experience a remarkable reduction in growth, yield, and reproduction. To expand our understanding of the molecular basis of the plant response to drought stress, the proteomic profile and protein-protein network of canola (<i>Brassica napus</i> L.) were studied. The focus was to show molecular mechanisms related to canola susceptibility to drought stress. The experiment used a completely randomized design, implemented in a hydroponic system under greenhouse conditions. To impose drought stress, plants were exposed to Hoagland’s solution supplemented with polyethylene glycol (PEG) 6000 for 7 days. The drought stress resulted in 161reproducible protein spots in twodimensional electrophoresis of canola leaves. The t-student test showed 21 differentially abundant proteins (DAP), of which 2 and 19 were up and down accumulated, respectively. Two spots identified as 1-aminocyclopropane-1-carboxylate oxidase and D-2-hydroxyglutarate dehydrogenase showed an increased abundance of 2.11 and 1.77, respectively. The extended protein-protein interaction of differentially abundant proteins and KEGG analysis showed 47 pathways directly and indirectly associated with canola response to drought stress. DAPs with increased abundance were associated with amino acid and signaling processes, whereas DAPs with decreased abundance were mostly connected with pathways responsible for energy production. The results of the study will help to elucidate further the molecular events associated with the susceptibility to drought stress in canola.
Highlights
The challenges of abiotic stress have widely been under attention as one of the major constraints to crop production
There are a little number of proteomics studies about canola-drought interactions and only a few have previously explored the proteomics profile of canola leaves under drought stress, mainly focused on tolerant cultivars and a little attention given on susceptible cultivars [25–27]
Previous findings have revealed that differentially abundant proteins (DAP) have a dynamic behavior and their abundance patterns depend on stress severity and duration, types of tissue, and growth stages [25–28]
Summary
The challenges of abiotic stress have widely been under attention as one of the major constraints to crop production. On the other hand, increasing human population and demand for more food exacerbate these challenges alongside other constraints such as climate change and biotic stress [1]. These challenges to secure food production for the world demand crops which are resilient to such conditions [1]. 2022 associated with response to abiotic stresses challenges the attempts to create tolerant cultivars [2]. This is on one hand because of the complexity of plant responses to abiotic stresses, and on the other hand because of lack of comprehensive information about the molecular basis of plant response [3]
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