Proteomic mechanisms for the combined stimulatory effects of glyphosate and antibiotic contaminants on Microcystis aeruginosa

Abstract

A single exposure to glyphosate or antibiotic may facilitate cyanobacterial growth at currently reported concentrations due to hormesis. However, the influence of these contaminants on cyanobacteria under combined exposure conditions has not been reported. In this study, proteomic mechanisms for the combined effects of glyphosate and a quaternary antibiotic mixture of amoxicillin, sulfamethoxazole, tetracycline, and ciprofloxacin in a dominant bloom-forming cyanobacterium (Microcystis aeruginosa) were investigated and compared with those for single exposure to glyphosate. The growth rate of M. aeruginosa, photosynthetic activity indicated by Fv/Fm, and microcystin production ability showed a typical U-shaped hormetic dose-response to glyphosate exposure. Upregulated proteins related to photosynthesis and biosynthesis, as well as increased photosynthetic activity, were responsible for the stimulated growth induced by 0.1–5 μg/L glyphosate, while the upregulation of mcyB protein contributed to increased microcystin synthesis in glyphosate-treated cells. The presence of 0.04–0.2 μg/L mixed antibiotics significantly (p < 0.05) enhanced the stimulation effects of glyphosate. Combined exposure to glyphosate and mixed antibiotics promoted microcystin synthesis through the upregulation of six microcystin synthesis regulatory proteins (mcyC, mcyF, mcyG, mcyI, MAE_56520, and ntcA) and stimulated cyanobacterial growth through the upregulation of proteins involved in photosynthesis, cell division, carbon fixation, pentose phosphate, translation, and chlorophyll synthesis. Combined exposure to glyphosate and antibiotic contaminants promoted cyanobacterial growth at no-effect concentrations of single exposure (0.04 μg/L for mixed antibiotics; 0.05, 10 and 100 μg/L for glyphosate), suggesting an increased threat from combined contamination to aquatic ecosystems through promoting the formation of cyanobacterial bloom.