Abstract
In budding yeast Saccharomyces cerevisiae, Snf1 (sucrose non‐fermenting) protein kinase of the Snf1/AMP‐activated protein kinase family is required for growth on non‐fermentable carbon sources and non‐preferred sugars. Three kinases, Elm1, Sak1 and Tos3, activate Snf1 by phosphorylation of its activation‐loop threonine in stress condition such as low glucose level. The absence of all three upstream kinases causes the Snf‐ phenotype. In this study, substantial role of each upstream kinase of Snf1 was explored by proteomic analysis of S. cerevisiae strains grown in glucose and glycerol. MALDI‐TOF analysis indicated that each protein kinase regulates expressions of peculiar targets even in non‐stress condition.
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