Abstract
The giant ciliate Stentor coeruleus (S. coeruleus) is a suitable model organism for studying morphogenesis and regeneration at the single-cell level. It contains a prominent structure on the anterior end of the cell, known as the oral apparatus (OA). OA can be induced to shed by urea treatment and then new OA regenerates via a series of defined morphological events and the cell resumes normal feeding activity. We identified OA constituents in S. coeruleus by mass spectrometry. A total of 882 OA-associated proteins were identified; the homologs of 181 of these are known OA constituents in other organisms. The expression pattern of OA-associated genes during regeneration was investigated using single-cell transcriptome sequencing. The expression of most OA-associated genes was high during regeneration, indicating their stable expression after OA shedding. We also identified OA-associated differentially expressed genes that may be involved in regulating OA reconstruction. In summary, this study gives preliminary insight into the molecular basis of OA in S. coeruleus.
Highlights
The giant ciliate Stentor coeruleus is a suitable model organism for studying morphogenesis and regeneration at the single-cell level
This information enabled us to hypothesize the architecture of oral apparatus (OA) of S. coeruleus WHEL
We have identified protein components of the OA and determined the temporal changes in their expression during OA regeneration in S. coeruleus WHEL
Summary
The giant ciliate Stentor coeruleus is a suitable model organism for studying morphogenesis and regeneration at the single-cell level. The anterior end of the cell is dominated by thousands of cilia organized into a ciliated band of membranelles, known as the oral apparatus (OA). This feeding organelle can be induced to shed by urea treatment. The OA regenerates via a series of defined morphological events and the cell resumes normal feeding activity. These defined events provide an ideal opportunity to identify the protein constituents of OA and their expression patterns in OA regeneration
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