Abstract
Semen contains several proteins that are important to fertilization and to identify reproductive failures. There are proteins that are specie-specific expressed, although differs among several breeds. This article provides experimental data describing the protein profile of seminal plasma and spermatozoa of four healthy purebred dogs: Golden Retriever (n=3), Bernese Mountain Dog (n=4), Great Dane (n=3), and Maremmano-Abruzzese Sheepdog (n=3), housed at São Paulo state, Brazil. Semen samples were collected by manual stimulation of the penis in a presence of a teaser bitch, when possible. The seminal plasma and sperm cells were separated by centrifugation and prepared for mass spectrometry. The gene ontology annotation of the proteins found is described. This is the first time that proteomic profile of the semen of purebred dogs is described. These data are a valuable resource to improve the biotechnologies of reproduction applied to canid species.
Highlights
Semen contains several proteins that are important to fertilization and to identify reproductive failures
Raw data Healthy purebred dogs (Golden Retriever, Bernese Mountain Dog, Maremmano-Abruzzese Sheepdog, Great Dane), from different kennels located at São Paulo state, Brazil
Anamnesis, clinical and reproductive evaluation of 13 purebred dogs [Golden Retriever (n = 3), Bernese Mountain Dog (n = 4), Great Dane (n = 3), and MaremmanoAbruzzese Sheepdog (n = 3)], from different kennels located at São Paulo state, were performed to assess their health condition and to discard any reproductive pathologies
Summary
Veterinary Medicine Canine reproduction Text Instruments: Q-Tof mass spectrometer City: Cotia and Botucatu (Bernese Mountain Dog), Sorocaba (Great Dane and Golden Retriever) and Itapira (Maremmano-Abruzzese Sheepdog) Country: Brazil Latitude and longitude (and GPS coordinates) for collected samples/data: Cotia: Latitude 23o 36’ 15’’ south, Longitude 46o 55’ 27’’ west Botucatu: Latitude 22o 53’ 25’’ south, Longitude: 48o 27’ 19’’ west Sorocaba: Latitude 23o 30’ 06’’ south, Longitude 47o 27’ 29’’ west Itapira: Latitude 22o 26’ 11’ south, Longitude 46o 49’ 20’’ west In a public repository Repository name: Mendeley Data Data identification number: doi:10.17632/739k25yj4s.4. This is the first time a comparative protein expression profile of the proteins found in the spermatozoa and seminal plasma of purebred dogs is described, which are very important to improve biotechnologies of reproduction in canid species and in human. These data can be useful for researchers, pharmaceutical industry and veterinarians. These data can be used to compare additional findings about proteomic profile of semen from another species
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