Proteomic characterization of low molecular weight allergens and putative allergen proteins in lentil (Lens culinaris) cultivars of Bangladesh

Abstract

Here, we present the proteome profiling of low-molecular weight (<50 kDa) proteins of seven different lentil cultivars developed by Bangladesh Agricultural Research Institute. A total of 2873 peptides corresponding to 180 unique proteins were identified wherein >24% of them were described lentil allergens. Comparative relative quantitation showed differences in protein abundance of major allergen proteins such as Len c 1.0101, Len c 1.0102, and lipid transfer proteins (LTPs), indicating qualitative and quantitative variations in allergen proteins in lentil cultivars. In this report, for the first-time, the amino acid sequence of LTPs in lentil has been confirmed by high resolution mass spectrometry. In addition, ideal peptides of Len c 1.0101, Len c 1.0102, and LTPs allergens were further determined with multiple reaction monitoring (MRM) analysis. Therefore, this data could provide a great resource for further development of targeted, proteomics-based assays for quantification of lentil allergens.