Abstract
BackgroundUsing human keratinocyte HaCaT cell line model, we screened for proteins that changed their content due to SDS exposure in non-toxic dose (25 μg/ml, as determined by the MTT assay and microscopic examination) during 48 h.MethodsThe altered level of proteins from HaCaT keratinocytes exposed to SDS was analyzed by LC-MS/MS approach and quantified using Progenesis LC software.ResultsThe Pathview map of 131 upregulated proteins was built, and enhancement of glycolysis/gluconeogenesis was found.ConclusionsThe results of our study admit the possibility of promotion of the cutaneous neoplasia and/or the peculiarity of the response of immortalized keratinocytes to the SDS treatment and provide new insights into possible role of SDS as integrator of diverse signaling that influence cell fate decisions.
Highlights
Skin diseases are one of the most common human illnesses
Cytotoxicity of sodium dodecyl sulphate (SDS) in HaCaT cells as determined by MTT and NRU assays Changes in viability of immortalized HaCaT keratinocytes when exposed to different doses of SDS for 48 h were studied using light microscopy, MTT assay, and neutral red dye (NR) uptake test
It was shown that HaCaT cell line is characterized by a dose-dependent decrease in viability when exposed to sodium dodecyl sulphate
Summary
Skin diseases are one of the most common human illnesses. The International Classification list of human disease contains more than 1000 skin or skin-related disorders. Widespread of skin diseases is caused with the variety of skin functions, the features of its structure, and exposure with a large number of external factors including dermatologic, cosmetic, and pharmaceutical products (Sprenger et al 2010). Contact dermatitis is one of the most common skin pathologies. The definition of contact dermatitis refers to any skin inflammation that occurs because of exposure to irritants (chemicals, mechanical, and physical) or allergens (Chiang et al 2012). Among the well-known irritants of the skin are detergents, i.e., sodium dodecyl sulphate (SDS), which is used as a component in. Using human keratinocyte HaCaT cell line model, we screened for proteins that changed their content due to SDS exposure in non-toxic dose (25 μg/ml, as determined by the MTT assay and microscopic examination) during 48 h
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