Proteomic changes in different growth periods of ginseng roots

Abstract

For the first time, proteomics and biochemical variables have been employed to unravel the growth strategies for the different root growth periods of ginseng (Panax ginseng CA May., Araliaceae). Enzymatic activities and cellular contents, except for starch, related to defence and metabolism were significantly increased in the slow-growth period but decreased in the fast-growth period. Proteomic characterisation by two-dimensional gel electrophoresis (2DE) showed 83 differentially expressed spots; 62 spots were up-regulated and 21 spots were down-regulated in the slow-growth period when compared to the fast-growth period. The identification of these spots indicated that the major groups of differential proteins were associated with energy metabolism (37%) and defence (17%), which was consistent with the changes observed in the biochemical measurements. These results clearly demonstrate that ginseng stores energy during its fast-growth period to promote root elongation, whereas it expends energy to improve the synthesis of secondary metabolites and stress resistance during its slow-growth period. The levels of many proteins were changed during the conversion period from fast to slow growth, providing new insights into ginseng proteome evolution. The proposed hypothetical model explains the interaction of metabolic proteins associated with the growth strategies of ginseng.