Abstract
Maize chlorotic mottle virus (MCMV) has been occurring frequently worldwide and causes severe yield losses in maize (Zea mays). To better investigate the destructive effects of MCMV infection on maize plants, isobaric tagging for relative and absolute quantitation (iTRAQ)-based comparative proteomic analysis was performed on MCMV infected maize cv. B73. A total of 972 differentially abundant proteins (DAPs), including 661 proteins with increased abundance and 311 proteins with reduced abundance, were identified in response to MCMV infection. Functional annotations of DAPs and measurement of photosynthetic activity revealed that photosynthesis was decreased, while the abundance of ribosomal proteins, proteins related to stress responses, oxidation-reduction and redox homeostasis was altered significantly during MCMV infection. Two DAPs, disulfide isomerases like protein ZmPDIL-1 and peroxiredoxin family protein ZmPrx5, were further analyzed for their roles during MCMV infection through cucumber mosaic virus-based virus-induced gene silencing (CMV-VIGS). The accumulation of MCMV was suppressed in ZmPDIL-1-silenced or ZmPrx5-silenced B73 maize, suggesting ZmPDIL-1 and ZmPrx5 might enhance host susceptibility to MCMV infection.
Highlights
Plants are generally exposed to diverse biotic and abiotic stresses that significantly affects plant growth and development
The results revealed that those differentially abundant proteins (DAPs) under maize chlorotic mottle virus (MCMV) infection could be divided into 115 different pathways
We performed isobaric tagging for relative and absolute quantitation (iTRAQ)-based quantitative proteomic analysis to investigate the responses of maize to MCMV infection
Summary
Plants are generally exposed to diverse biotic and abiotic stresses that significantly affects plant growth and development. Extensive studies of comparative transcriptional and proteomic analysis revealed the roles of transcriptional reprogramming of defense genes and posttranslational modification of proteins in plant immunity to pathogens [2,8,9,10]. Several RNA viruses—such as sugarcane mosaic virus (SCMV), rice black streaked dwarf (RBSDV), maize dwarf mosaic virus (MDMV), maize rough dwarf virus (MRDV), and maize chlorotic mottle virus (MCMV)—have been reported as common viral agents infecting maize [11,12,13]. Synergistic interactions of MCMV with potyviruses, such as wheat streak mosaic rymovirus or SCMV, are fairly common in maize fields [13,14]
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