Proteomic and Transcriptomic Analysis of Grapevine PR10 Expression During Salt Stress and Functional Characterization in Yeast

Abstract

Proteomic and transcriptomic analyses of pathogenesis-related proteins, PR10, were carried out by subjecting adult plants of the salt-tolerant grapevine cv. Razegui to 100 mM NaCl for 6 and 24 h under controlled greenhouse conditions. Within 6 h of salt exposure, an increase in levels of RzPR10 was observed in the roots. PR10 mRNA levels in leaves were significantly different than those in roots; moreover, significant differences for mRNA levels were found between treated and control plants. Within 24 h of salt treatment, roots exhibited the highest levels of RzPR10 protein, but no differences were observed for mRNA levels in both leaves and roots. Overexpression of Vvpr10 cDNA, under the control of the Gal promoter, in the yeast Saccharomyces cerevisiae revealed that growth of transformed cells on a minimal medium containing 500 mM NaCl is similar to that of cells cultivated on NaCl-free medium. This indicated that Vvpr10 gene was functional in yeast and conferred tolerance to high salt concentrations.