Abstract
Seeds lose their viability when they are exposed to high temperature and moisture content (MC) during storage. The expression and metabolism of proteins plays a critical role in seed resistance to heat stress. However, the proteome response to heat stress in oat (Avena sativa) seeds during storage has not been revealed. To understand mechanisms of heat stress acclimation and tolerance in oat seeds, an integrated physiological and comparative proteomic analysis was performed on oat seeds with different MC during heat stress. Oat seeds with 10% and 16% MC were subjected to high temperatures (35, 45, and 50°C) for 24 and 2 days, respectively, and changes in physiological and biochemical characteristics were analyzed. The results showed that seed vigor decreased significantly with temperature increase from 35 to 50°C. Also, the proline content in 10% MC seeds decreased significantly (p < 0.05) whereas that in 16% MC seeds increased significantly (p < 0.05) during heat treatment from 35 to 50°C. There were no significant differences in malondialdehyde content in 10% MC seeds with temperature from 35 to 50°C, but a significant (p < 0.05) decline occurred in 16% MC seeds at 45°C. Proteome analysis revealed 21 significantly different proteins, including 19 down-regulated and two up-regulated proteins. The down-regulated proteins, notably six heat shock proteins and two ATP synthases, have important roles in the mobilization of carbohydrates and energy, and in the balance between synthesis and degradation of other proteins during seed deterioration. The up-regulation of argininosuccinate synthase participated in proline biosynthesis at 16% MC, which is important for maintaining reactive oxygen species homeostasis for the resistance of heat stress. In summary, heat-responsive protein species and mitochondrial respiratory metabolism were sensitive to high temperature and MC treatment. These studies provide a new insight into acclimation and tolerance to heat stress in oat seeds.
Highlights
Crop seeds may deteriorate and lose their ability to germinate following storage at elevated temperatures and for extended periods of time
The germination percentage of oat seeds showed a trend toward decrease with temperature increase from 35 to 50◦C (Figure 1A)
The decrease of seed germination percentage and germination index in oat seeds with 10 or 16% moisture content (MC) as temperature was raised from 45 to 50◦C confirmed the occurrence of Seed deterioration (SD)
Summary
Crop seeds may deteriorate and lose their ability to germinate following storage at elevated temperatures and for extended periods of time. SD occurs during prolonged storage and escalates when seeds are stored under improper conditions, especially at high temperature and moisture content (MC) (McDonald, 1999; Arc et al, 2011). In agriculture, deteriorated seed germinates poorly and negatively affects seedling growth and yield. Low temperature and MC were helpful in prolonging storage lifespan (Walters et al, 2005), while high temperature and humidity induced and accelerated the SD process (El-Maarouf-Bouteau et al, 2011). The effects of increased seed MC and storage temperature, or the mutual relationship between them, on the deterioration process needs to be clarified
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