Proteomic and lipidomic analysis of exosomes derived from ovarian cancer cells and ovarian surface epithelial cells

Lin Cheng,Peng Jin,Manhua Cui,Kun Zhang,Yunan Qing,Tianmin Xu,Dong Li

doi:10.1186/s13048-020-0609-y

Abstract

BackgroundThe limitation of current biomarker of early stage ovarian cancer and the anatomical location of ovarian (depths of the pelvic) make ovarian cancer difficult to be detected in early stage. Growing evidence shows exosomes as key information transmitters, it carried molecules, such as miRNAs, proteins, lipids, double-stranded DNA have been reported as promising biomarkers in many diseases. However, little is known about the protein and lipid composition of ovarian cancer.MethodsHere, we report proteomic and lipidomic analysis of exosomes derived from ovarian cancer cells (SKOV-3) and ovarian surface epithelial cells (HOSEPiC).ResultsA total of 1433 proteins and 1227 lipid species were identified from two cell line derived exosomes. Several lipid species and proteins significantly differ in SKOV-3 derived exosomes compared to those from HOSEPiC. For example, we noted that ChE and ZyE species were in general more abundant in exosomes from SKOV-3 than from HOSEPiC; Collagen type V alpha 2 chain (COL5A2) and lipoprotein lipase (LPL) were significantly higher in SKOV-3 derived exosomes than HOSEpic (p < 0.05).ConclusionsOur research indicates the promising role of exosomal proteins and lipids in the early diagnosis of ovarian cancer.

Highlights

The limitation of current biomarker of early stage ovarian cancer and the anatomical location of ovarian make ovarian cancer difficult to be detected in early stage
Cell culture An human ovarian surface epithelial cell line (HOSEPiC) and an ovarian cancer cell SKOV-3 were cultured in 37 °C in 5% carbon dioxide and RMPI-1640 medium supplemented with 10 and 15%, EV-depleted fetal bovine serum (FBS was centrifuged overnight at 120,000 g to pellet out vesicles), 100 units/ml penicillin and 100 μg/ ml streptomycin
Characterization of exosomes derived from two ovarian cell lines The exosomes secreted by SKOV-3 and HOSEPiC cells were isolated from EV-depleted medium by a combination of differential centrifugation

Summary

Introduction

The limitation of current biomarker of early stage ovarian cancer and the anatomical location of ovarian (depths of the pelvic) make ovarian cancer difficult to be detected in early stage. Growing evidence shows exosomes as key information transmitters, it carried molecules, such as miRNAs, proteins, lipids, double-stranded DNA have been reported as promising biomarkers in many diseases. The mechanism of exosome biogenesis and cargo selection are still unclear, but there are several studies provided precise clues [4,5,6,7,8]. Exosomes exchange information with distant cells via carrying complex packets stuffed with a selection of proteins, lipids, and nucleic acids. Exosomes seem as a vehicle that is full of ideal non-invasive biomarkers with great potential in the detection of oncogenesis, tumor spread, and drug resistance. Data attributing to cancer specific intercellular transfer molecules to exosomes are still limited. In-depth characterization of exosomes will help to elucidate their precise biological functions

Methods

Results

Conclusion