Proteomic analysis using SELDI-TOF in cervical and amniotic fluid to detect biomarkers released in response to intra-amniotic inflammation

Abstract

DETECT BIOMARKERS RELEASED IN RESPONSE TO INTRA-AMNIOTIC INFLAMMATION BO JACOBSSON, ULLA RUESCHI, ASA ROSEN, LARS RYMO, HENRIK HAGBERG, Perinatal Center, Dept Obstetrics and Gynecology, Goteborg, Sweden, Department of Clinical Chemistry and Transfusion Medicine, Department of Clinical Chemistry and Transfusion Medicine, Goteborg, Sweden OBJECTIVE: There is an urgent need to understand the pathophysiological mechanisms behind preterm birth in order to develop therapeutic and preventive strategies. Lately, evidence has grown strong for an association between intrauterine inflammation (IAI) and preterm birth in women with preterm labor (PTL) or preterm premature rupture of membranes (pPROM). In that perspective identification of novel biomarkers is of great importance. Our objective was to use a proteomic strategy, SELDI-TOF-MS, to determine whether protein profiling could reveal novel biomarkers present in cervical and amniotic fluid of PTL/pPROM patients with IAI (defined as elevated amniotic IL-6) compared to non-IAI cases. STUDY DESIGN: The study-material included amniotic fluid samples from 27 individuals with either PTL (n = 13) or pPROM (n = 14) before 34 weeks of gestation. Cervical fluid were present from those in PTL. Fourteen of the 27 women had IAI defined as elevated concentrations of IL-6 in amniotic fluid. Cervical and amniotic fluid proteins were analyzed by SELDI-TOF-MS using a strong anion array surface (Q 10) using sinapinic acid as the energy-absorbing molecule. RESULTS: Protein profiles revealed large individual variation in the amniotic fluid protein composition. However, we detected five peaks that were strongly over-expressed (P ! .0002) in amniotic fluid in IAI samples compared non-IAI samples. Four of the peaks were detected in the mass range 3400-4100 Da, the fifth peak was detected at 24 600 Da. The difference was not present regarding these five peaks in cervical fluid. CONCLUSION: Preliminary data suggest that novel biomarkers could be detected with proteomic analysis using SELDI-TOF and these proteins might S108 SMFM Abstracts