Abstract
The distribution of differential extracellular matrix (ECM) in the lateral and medial menisci can contribute to knee instability, and changes in the meniscus tissue can lead to joint disease. Thus, deep proteomic identification of the lateral and medial meniscus cartilage is expected to provide important information for treatment and diagnosis of various knee joint diseases. We investigated the proteomic profiles of 12 lateral/medial meniscus pairs obtained from excess tissue of osteoarthritis patients who underwent knee arthroscopy surgery using mass spectrometry-based techniques and measured 75 ECM protein levels in the lesions using a multiple reaction monitoring (MRM) assay we developed. A total of 906 meniscus proteins with a 1% false discovery rate (FDR) was identified through a tandem mass tag (TMT) analysis showing that the lateral and medial menisci had similar protein expression profiles. A total of 131 ECM-related proteins was included in meniscus tissues such as collagen, fibronectin, and laminin. Our data showed that 14 ECM protein levels were differentially expressed in lateral and medial lesions (p < 0.05). We present the proteomic characterization of meniscal tissue with mass spectrometry-based comparative proteomic analysis and developed an MRM-based assay of ECM proteins correlated with tissue regeneration. The mass spectrometry dataset has been deposited to the MassIVE repository with the dataset identifier MSV000087753.
Highlights
The knee is the largest hinge joint associated with weight-bearing and movement in the human body
Nine proteins consisting of collagen alpha1(XVIII) chain (COL18A1), Cystatin-B (CSTB), Cathepsin D (CTSD), Cathepsin Z (CTSZ), Protein ERGIC-53 (LMAN1), Protein S100-A13 (S100A13), Adiponectin (ADIPOQ), Alpha1-antichymotrypsin (SERPINA3), and secreted protein acidic and rich in cysteine (SPARC)-related modular calcium-binding protein 2 (SMOC2) had greater expression in the medial lesion compared to the lateral (Figure 1A)
Several studies have profiled the proteome of articular cartilage and medial meniscus tissue, but the extracellular matrix (ECM) profile in these tissues is not sufficiently covered and quantified [8,9]
Summary
The knee is the largest hinge joint associated with weight-bearing and movement in the human body. The knee contains medial and lateral menisci; the medial meniscus tends to experience more frequent damage than the lateral meniscus due to its anatomical structure and articular mechanism [1]. Such damage includes a tear, which occurs when placing excessive pressure on or twisting the knee joint. Pairwise comparisons of the medial/lateral meniscus is a potentially unique model for the study of cartilage proteomics in osteoarthritis genesis and progression, because the anatomical structure and biomechanical configuration of the knee is typically associated with much more severe cartilage loss on the predominantly weight-bearing medial compartment. We investigated the proteome profile of meniscal tissue of lateral and medial lesions and characterized the extracellular matrix proteins. We provide extracellular matrix data and use multiple reaction monitoring (ECM-MRM) assays to compare expression levels in two lesion types
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