Abstract
This chapter discusses the proteomic analysis study of the mammalian cell nucleus. The proteome consists of all proteins present in a cell or organism at a given time, including not only those translated directly from genetic material, but also the variety of modified proteins arising from events, such as alternative splicing of transcripts and extensive posttranslational processing. Proteomics provides valuable information and correlates genome sequence information and the cellular behavior at the molecular level. In this chapter, approaches to perform proteomics of the mammalian cell nucleus are discussed. Major steps involve separation and identification of nuclear proteins using two-dimensional gel electrophoresis (2DE) and mass spectrometry (MS) followed by data analysis and interpretation using advanced bioinformatic techniques. Nuclear protein solubilization for sample preparation, isoelectric focusing of protein, concepts related to visualization of protein spots by staining 2D gels, details of coomassie brilliant blue R-250 staining, sypro ruby protein gel stain (Bio-Rad), and mass spectrometry compatible silver staining, andAn image analysis of two-dimensional gels are described.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
