Abstract
BackgroundClassical swine fever virus (CSFV) belongs to the genus Pestivirus within the family Flaviviridae. Virulent strains of classical swine fever virus (CSFV) cause severe disease in pigs characterized by immunosuppression, thrombocytopenia and disseminated intravascular coagulation, which causes significant economic losses to the pig industry worldwide.MethodsTo reveal proteomic changes in swine serum during the acute stage of lethal CSFV infection, 5 of 10 pigs were inoculated with the virulent CSFV Shimen strain, the remainder serving as uninfected controls. A serum sample was taken at 3 days post-infection from each swine, at a stage when there were no clinical symptoms other than increased rectal temperatures (≥40°C). The samples were treated to remove serum albumin and immunoglobulin (IgG), and then subjected to two-dimension differential gel electrophoresis.ResultsQuantitative intensity analysis revealed 17 protein spots showing at least 1.5-fold quantitative alteration in expression. Ten spots were successfully identified by MALDI-TOF MS or LTQ MS. Expression of 4 proteins was increased and 6 decreased in CSFV-infected pigs. Functions of these proteins included blood coagulation, anti-inflammatory activity and angiogenesis.ConclusionThese proteins with altered expression may have important implications in the pathogenesis of classical swine fever and provide a clue for identification of biomarkers for classical swine fever early diagnosis.
Highlights
Classical swine fever virus (CSFV) belongs to the genus Pestivirus within the family Flaviviridae
Studies have shown that cytokines released from monocytes/macrophages activated by CSFV infection
Comparative proteomic analysis of CSFV-infected and uninfected serum samples Serum proteomic profiles of CSFV-infected and uninfected pigs were analyzed by 2-D difference gel electrophoresis (DIGE)
Summary
Classical swine fever virus (CSFV) belongs to the genus Pestivirus within the family Flaviviridae. Virulent strains of classical swine fever virus (CSFV) cause severe disease in pigs characterized by immunosuppression, thrombocytopenia and disseminated intravascular coagulation, which causes significant economic losses to the pig industry worldwide. CSF caused by virulent strains of CSFV is a hemorrhagic disease of pigs, characterized by disseminated intravascular coagulation, thrombocytopenia and immunosuppression. Apart from above factors that contribute to the pathogenesis and progression of CSF, little is known of changes in serum proteins and biomarker for diagnosis and prognosis of the disease. Growing interest has been focused on the changes in serum protein expression in experimental virus infections to found sera proteins concerning pathogenesis or biomarker for diagnosis or prognosis. Thereby, analysis of the profile of serum protein alterations is a promising way to try finding potential biomarker and highlighting the pathogenesis of disease
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