Abstract
BackgroundCotton worm is one of the main insects of soybean in southern China. Plants may acquire defense mechanisms that confer protection from predation by herbivores. Induced responses can lead to increased resistance against herbivores in many species. This study focuses on searching changed proteins in soybean defense response induced by cotton worm feeding.ResultsTen protein spots that are changed in abundance in response to cotton worm feeding were identified by Two-dimensional gel electrophoresis (2-DE). A total of 11 unique proteins from these spots were identified by MALDI-TOF MS. The mRNA and protein relative expression levels of most changed proteins were up-regulated. These proteins were mainly involved in physiological processes, including active oxygen removal, defense signal transduction, and metabolism regulation.ConclusionThis is the first proteomic analysis of the soybean defense response induced by cotton worm. The differentially expressed proteins could work together to play a major role in the induced defense response. PAL and SAMS were up-regulated at both the protein and mRNA levels. These genes can be strongest candidates for further functional research.
Highlights
Cotton worm is one of the main insects of soybean in southern China
Induction of soybean defense response by cotton worm feeding To determine whether cotton worm feeding can induce resistance in soybean leaves, we carried out a dual-choice test and force-feeding experiment (Figure 1)
In the force-feeding experiment, the relative growth rates of cotton worms feeding on control leaves and those feeding on treated leaves were similar
Summary
Cotton worm is one of the main insects of soybean in southern China. Plants may acquire defense mechanisms that confer protection from predation by herbivores. Induced responses can lead to increased resistance against herbivores in many species. This study focuses on searching changed proteins in soybean defense response induced by cotton worm feeding. Insect pests can adversely affect the yield and quality of soybean. Cotton worm is one of the main insect pests of soybean in southern China [3,4]. Improvement in resistance to cotton worm is one of the main soybean breeding objectives. During the process of evolution, plants have acquired defense mechanisms that confer protection from predation by herbivores. Two modes of resistance to herbivores exist: constitutive resistance, which is expressed
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