Abstract
In response to injury, dental pulp cells differentiate into odontoblast-like cells to produce reparative dentin. To understand the mechanism of odontoblastic differentiation and to stimulate reparative dentin formation, we developed an in vitro culture system representing odontoblastic differentiation of bovine dental pulp cells and profiled the protein expression through proteomic analyses. Fractionation with which two-dimensional gel electrophoresis exhibited an analogous protein expression pattern between in vivo and in vivo pulp cells, but underlined differentially expressed proteins during the odontoblastic differentiation. Subsequent mass spectrometry and NH2-terminal amino acid sequence demonstrated degradation of vimentin into several species, up-regulation of connexin 43 and down-regulation of protein disulfide isomerase in the differentiating pulp cells and suggests the involvement of these proteins in odontoblastic differentiation.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
