Abstract
Differential analysis of outer membrane composition of S. Enteritidis strains, resistant to 50% normal human serum (NHS) was performed in order to find factors influencing the resistance to higher concentrations of NHS. Ten S. Enteritidis clinical strains, resistant to 50% NHS, all producing very long lipopolysaccharide, were subjected to the challenge of 75% NHS. Five extreme strains: two resistant and three sensitive to 75% NHS, were chosen for the further analysis of outer membrane proteins composition. Substantial differences were found in the levels of particular outer membrane proteins between resistant and sensitive strains, i.e. outer membrane protease E (PgtE) was present mainly in resistant strains, while sensitive strains possessed a high level of flagellar hook-associated protein 2 (FliD) and significantly higher levels of outer membrane protein A (OmpA).
Highlights
For many years bacteria from the genus of Salmonella have remained the leading cause of gastrointestinal diseases, posing a significant epidemiological threat to the community
As C3 is a crucial protein in the activation of the complement cascade, standard analysis of C3 protein levels in normal human serum (NHS) used for experiments were performed
Enteritidis were found to be resistant to NHS in the standard test of the bactericidal activity with 50% NHS (Table 2)
Summary
For many years bacteria from the genus of Salmonella have remained the leading cause of gastrointestinal diseases, posing a significant epidemiological threat to the community. Every year 94 million infections, caused by nontyphoidal Salmonella (NTS) organisms, are recorded worldwide [1]. These epidemiological data are probably highly underestimated [2], as most salmonellosis cases are not reported due to the short course of the disease and no hospitalization. Typhimurium) are the two most common clinical serovars (serological variants) isolated worldwide as causative agents of salmonellosis [1]. Typhimurium (65% and 12% of all isolates worldwide, respectively) [4,5]
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