Abstract
Hypertensive damage in the non‐clipped kidney of two‐kidney, one‐clip (2K1C) rats is most pronounced in the juxtamedullary cortex (JMC), while the outer cortex (OC) is protected. In this study, 2K1C was induced with a 0.2mm silver clip in 6‐week‐old male Wistar rats that were sacrificed after 16‐weeks and compared to sham operated controls (19‐weeks). Differentially abundant proteins were identified in a hypothesis free manner using trypsin digested OC and JMC that was tagged with iTRAQ‐4plex, and analyzed on an Orbitrap Velos Pro liquid‐chromatography tandem mass spectrometer.2714 proteins were quantified (FDR < 0.01). 269 proteins increased, and 127 decreased in abundance in 2K1C compared to control (fold change >; 1.5). The number of differential abundant proteins was higher in JMC (338) than in OC (231). 51 of the differential abundant proteins overlapped with 565 differentially expressed genes from microarray gene expression data obtained previously in the same model.In conclusion, hypertensive kidney damage in the non‐clipped kidney of 2K1C is associated with large changes in the proteome, which are more pronounced in the JMC. Important proteins identified include Osteopontin, S100A4 and Decorin, which are known to play a role in fibrosis and tissue remodeling.This work was funded by Western Norway Regional Health Authority and the University of Bergen.
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