Abstract
ABSTRACT Meiosis is the process by which haploid gametes are produced from diploid precursor cells. We used stable isotope labeling by amino acids in cell culture (SILAC) to characterize the meiotic proteome in the fission yeast Schizosaccharomyces pombe. We compared relative levels of proteins extracted from cells harvested around meiosis I with those of meiosis II, and proteins from premeiotic S phase with the interval between meiotic divisions, when S phase is absent. Our proteome datasets revealed peptides corresponding to short open reading frames (sORFs) that have been previously identified by ribosome profiling as new translated regions. We verified expression of selected sORFs by Western blotting and analyzed the phenotype of deletion mutants. Our data provide a resource for studying meiosis that may help understand differences between meiosis I and meiosis II and how S phase is suppressed between the two meiotic divisions.
Highlights
Sexual reproduction depends on meiosis, a process that generates haploid gametes from a diploid precursor cell
Our data provide a resource for studying meiosis that may help understand differences between meiosis I and meiosis II and how S phase is suppressed between the two meiotic divisions
Progression of meiosis is accompanied by complex changes of gene expression [5]. These changes in fission yeast meiosis have been studied by various approaches including transcriptional profiling using DNA microarrays and ribosome profiling to investigate the translational landscape [6, 7]
Summary
Sexual reproduction depends on meiosis, a process that generates haploid gametes from a diploid precursor cell. One of the advantages of using fission yeast is that highly synchronous meiosis can be induced by inactivation of the Pat1 protein kinase [1,2,3,4]. A comprehensive study analyzing changes of the S. pombe meiotic proteome using stable isotope labeling by amino acids in cell culture (SILAC) was published during the course of our work [8].
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