Abstract
Humoral immune components have been individually studied in the context of interaction of host with Aspergillus fumigatus, a major airborne fungal pathogen. However, a global view of the multitude and complex nature of humoral immune components is needed to bring new insight into host-Aspergillus interaction. Therefore, we undertook comparative proteomic analysis of the bronchoalveolar lavage fluid collected from individuals infected or colonized with A. fumigatus versus controls, to identify those alveolar humoral components affected upon A. fumigatus infection. Complement proteins C1q, C8 beta-chain, factor-H, ficolin-1, ficolin-2, mannan binding lectin serine peptidase 2, pentraxin-3 and the surfactant protein-D were identified as the major humoral immune components affected by A. fumigatus infection and colonization. Based on this observation, we hypothesize that crosstalk between these humoral components is essential during host-Aspergillus interaction giving new specific leads to study for better understanding the pathogenesis. Furthermore, the affected humoral components could be potential diagnostic markers of A. fumigatus infection or colonization.
Highlights
Aspergillus fumigatus, an airborne fungal pathogen, causes a range of allergic entities to invasive and chronic infections in patients with immune disorders and/or underlying pulmonary dysfunctions [1]
A total of 518 proteins were less abundant in the Aspergillus+ bronchoalveolar lavage fluid (BALF) than in the control, among which 337 proteins were absent in the Aspergillus+ BALF (Figure 1A)
Humoral immune components that were completely absent in the Aspergillus+ BALF were C1q subunitA, mannan binding lectin serine peptidase-2 (MASP2), ficolin-2 (FCN2), complement factor H related proteins 2 and 5 (CFHR2 and CFHR5), complement-8 (C8) beta-chain, C-type lectin receptor CD206 and surfactant protein D (SP-D)
Summary
Aspergillus fumigatus, an airborne fungal pathogen, causes a range of allergic entities to invasive and chronic infections in patients with immune disorders and/or underlying pulmonary dysfunctions [1]. The morbidity and mortality rate due to aspergillosis, the infection caused by A. fumigatus, remains high, which could be partly because of our poor knowledge on immunobiology of this fungus. The cellular immune system against A. fumigatus is well studied, the role of humoral immune system against this fungus is an underexplored and/or a. Conidial interaction with bronchoalveolar lavage fluid (BALF) or serum showed significant difference in the humoral immune components interacting and subsequent immune response [3]. This justifies the use of BALF in identifying major players of humoral immunity against A. fumigatus
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