Abstract
BackgroundThe vitreous humor is a transparent, gelatinous mass whose main constituent is water. It plays an important role in providing metabolic nutrient requirements of the lens, coordinating eye growth and providing support to the retina. It is in close proximity to the retina and reflects many of the changes occurring in this tissue. The biochemical changes occurring in the vitreous could provide a better understanding about the pathophysiological processes that occur in vitreoretinopathy. In this study, we investigated the proteome of normal human vitreous humor using high resolution Fourier transform mass spectrometry.ResultsThe vitreous humor was subjected to multiple fractionation techniques followed by LC-MS/MS analysis. We identified 1,205 proteins, 682 of which have not been described previously in the vitreous humor. Most proteins were localized to the extracellular space (24%), cytoplasm (20%) or plasma membrane (14%). Classification based on molecular function showed that 27% had catalytic activity, 10% structural activity, 10% binding activity, 4% cell and 4% transporter activity. Categorization for biological processes showed 28% participate in metabolism, 20% in cell communication and 13% in cell growth. The data have been deposited to the ProteomeXchange with identifier PXD000957.ConclusionThis large catalog of vitreous proteins should facilitate biomedical research into pathological conditions of the eye including diabetic retinopathy, retinal detachment and cataract.
Highlights
The vitreous humor is a transparent, gelatinous mass whose main constituent is water
We identified several members of the insulin-like growth factor-binding protein (IGFBP) family including IGFBP2, IGFBP4, insulinlike growth factor 2 (IGF2), insulin-like growth factor binding protein acid labile subunit (IGFALS)
Of the 682 novel proteins, we identified proteins such as S-Arrestin(SAG) and transforming growth factor-beta 2 (TGF-B2) which are involved in the pathophysiology of ocular diseases
Summary
The vitreous humor is a transparent, gelatinous mass whose main constituent is water. It plays an important role in providing metabolic nutrient requirements of the lens, coordinating eye growth and providing support to the retina. We investigated the proteome of normal human vitreous humor using high resolution Fourier transform mass spectrometry. The vitreous is a highly hydrated gelatinous mass that fills the space between the lens and the retina. The space between the two is the future acts as a metabolic repository for the retina, hyalocytes and surrounding tissues [3], some of the proteins in vitreous humor could be contributed by these surrounding tissues. Of the soluble proteins that constitute the vitreous, sialic acid containing glycoproteins constitute the largest fraction [10]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
