Abstract
BackgroundHuman follicular fluid (HFF) provides a key environment for follicle development and oocyte maturation, and contributes to oocyte quality and in vitro fertilization (IVF) outcome.MethodsTo better understand folliculogenesis in the ovary, a proteomic strategy based on dual reverse phase high performance liquid chromatography (RP-HPLC) coupled to matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (LC-MALDI TOF/TOF MS) was used to investigate the protein profile of HFF from women undergoing successful IVF.ResultsA total of 219 unique high-confidence (False Discovery Rate (FDR) < 0.01) HFF proteins were identified by searching the reviewed Swiss-Prot human database (20,183 sequences), and MS data were further verified by western blot. PANTHER showed HFF proteins were involved in complement and coagulation cascade, growth factor and hormone, immunity, and transportation, KEGG indicated their pathway, and STRING demonstrated their interaction networks. In comparison, 32% and 50% of proteins have not been reported in previous human follicular fluid and plasma.ConclusionsOur HFF proteome research provided a new complementary high-confidence dataset of folliculogenesis and oocyte maturation environment. Those proteins associated with innate immunity, complement cascade, blood coagulation, and angiogenesis might serve as the biomarkers of female infertility and IVF outcome, and their pathways facilitated a complete exhibition of reproductive process.
Highlights
Human follicular fluid (HFF) provides a key environment for follicle development and oocyte maturation, and contributes to oocyte quality and in vitro fertilization (IVF) outcome
Identification of high-confidence HFF proteome by dual reverse phase high performance liquid chromatography (RP-HPLC) coupled with MALDI Matrix-assisted laser desorption/ionization time-of-flight tandem (TOF/TOF) mass spectrometry
A peptide sequencing strategy was applied by using two-dimensional chromatography-MALDI TOF/TOF mass spectrometry
Summary
Human follicular fluid (HFF) provides a key environment for follicle development and oocyte maturation, and contributes to oocyte quality and in vitro fertilization (IVF) outcome. In vitro fertilization (IVF) coupled with embryo transfer into uterus has been applied as treatment for infertility several decades. HFF provided a special milieu to facilitate the communications between occyte and follicular cells, the development of follicle and the maturation of occytes. The alteration of HFF proteins reflected disorders of main secretary function of granulosa cells and thecae, and the damage of blood follicular barrier, which was associated with abnormal folliculogenesis [9] and a diminished reproductive potential [10]. In IVF treatment, HFF was accessible during the aspiration of oocytes from follicle, and was an ideal source for noninvasive screening of biomakers for oocyte maturation, fertilization success, IVF outcome, pregnancy, and ovarian diseases
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
