Proteomic analysis of elite soybean Jidou17 and its parents using iTRAQ-based quantitative approaches

Jun Qin,Jianan Zhang,Shuangjin Zhao,Duan Liu,Changcheng Yin,Hao Chen,Chunyan Yang,Feng Gu,Mengchen Zhang,Xu Zhan

doi:10.1186/1477-5956-11-12

Jun Qin, Jianan Zhang + Show 8 more

Open Access

https://doi.org/10.1186/1477-5956-11-12

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Abstract

BackgroundDerived from Hobbit as the female parent and Zao5241 as the male parent, the elite soybean cultivar Jidou17 is significantly higher yielding and shows enhanced qualities and stronger resistance to non-biological stress than its parents. The purpose of this study is to understand the difference in protein expression patterns between Jidou17 and its parental strains and to evaluate the parental contributions to its elite traits.ResultsLeaves (14 days old) from Jidou17 and its parental cultivars were analysed for differential expressed proteins using an iTRAQ-based (isobaric tags for relative and absolute quantitation) method. A total of 1269 proteins was detected, with 141 and 181 proteins in Jidou17 differing from its female and male parent, respectively. Functional classification and an enrichment analysis based on biological functions, biological processes, and cellular components revealed that all the differential proteins fell into many functional categories but that the number of proteins varied greatly for the different categories, with enrichment in specific categories. A pathway analysis indicated that the differentiated proteins were mainly classified into the ribosome assembly pathway. Protein expression clustering results showed that the expression profiles between Jidou17 and its female parent Hobbit were more similar than those between Jidou17 and its male parent Zao5241 and between the two parental strains. Therefore, the female parent Hobbit contributed more to the Jidou17 genotype.ConclusionsThis study applied a proven technique to study proteomics in 14-day-old soybean leaves and explored the depth and breadth of soybean protein research. The results provide new data for further understanding the mechanisms of elite cultivar development.

Highlights

Traditional plant breeders developing new cultivars mainly rely on phenotypes, which, for soybean breeding, often include such agricultural characteristics as growth, biomass, yield, resistance to disease, and tolerance to environmental stress
Proteomic analysis The iTRAQ method was applied for the proteomic analysis of 14-day-old leaves from Jidou17, its female parent (Hobbit), and its male parent (Zao5241)
The proteins that were differentially expressed between Jidou17 and its parental strains were classified into seven categories (Table 1)

Summary

Introduction

Traditional plant breeders developing new cultivars mainly rely on phenotypes, which, for soybean breeding, often include such agricultural characteristics as growth, biomass, yield, resistance to disease, and tolerance to environmental stress. Soybean is an important economic crop, and the selection of filial generations during breeding is conducted based on the evaluation of important agronomic traits. Exploration of the development of different phenotypes and differential protein expression patterns could reveal some of the mechanisms involved in cultivar development. Derived from Hobbit as the female parent and Zao5241 as the male parent, the elite soybean cultivar Jidou is significantly higher yielding and shows enhanced qualities and stronger resistance to non-biological stress than its parents. The purpose of this study is to understand the difference in protein expression patterns between Jidou and its parental strains and to evaluate the parental contributions to its elite traits

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Conclusion