Abstract
BackgroundGlucohexaose is a safe farm chemical used for pathogen prevention, which can induce systemic acquired resistance in cucumber.ResultsWe found that glucohexaose treatment of cucumber plant induced an accumulation of the reactive oxidative species (ROS). Histochemistry showed sharp increases in O2- and H2O2 5 h after glucohexaose treatment. After 5 h, the O2- content decreased to a normal level, but the H2O2 content remained at a high level 10 h after glucohexaose treatment. And antioxidant enzymes were also changed after glucohexaose treatment. We also investigated the relationship between ROS accumulation and glucohexaose-induced proteome alteration using 2D electrophoresis coupled with MS/MS. 54 protein spots, which enhanced expression under glucohexaose treatment but suppressed the expression by application of DPI and DMTU, have been identified.ConclusionOur study showed the accumulation of ROS is a part of mechanism of glucohexaose induced resistance in cucumber cotyledons. The up-regulated proteins identified by MS such as PP2C and antioxidation proteins are important in ROS signaling. It will be interesting to find out the regulatory mechanism underlying the induction of these proteins via ROS, and provide some clues to the mechanism of glucohexaose-induced resistance.
Highlights
During vegetable production, chemical pesticides are still the main method of disease prevention
We report that glucohexaose can induce reactive oxidative species (ROS) accumulation in cucumber cotyledons and provide some clues concerning the mechanism of glucohexaoseinduced ROS accumulation
Glucohexaose-induced ROS accumulation To determine whether glucohexaose can induce an ROS accumulation in cucumber cells and when the accumulation occurs, we detected two ROS, Hydrogen peroxide (H2O2) and O2−, using DAB and NBT staining at 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14 and 15 h after glucohexaose treatment
Summary
Chemical pesticides are still the main method of disease prevention. As consumers’ concerns about food quality increase, how to prevent disease without pesticide residues has attracted more attention. Induced resistance by biotic and abiotic elicitors is a new method for disease resistance. Glucohexaose, synthesized by the Research Center for Eco-Environmental Science, Chinese Academy of Science, is a safe, synthetic oligosaccharide elicitor that is naturally degraded in the environment. Plant resistance systems were activated and plants acquired stronger resistance to many pathogens, such as Pseudoperonospora cubensis [1]. Glucohexaose is a safe farm chemical used for pathogen prevention, which can induce systemic acquired resistance in cucumber
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