Proteomic Analysis of Compatible Interaction between Sorghum Downy MildewPathogen (Peronosclerospora Sorghi) and Maize (Zea MaysL.)

Abstract

Proteomic Analysis of Compatible Interaction between Sorghum Downy Mildew Pathogen (Peronosclerospora Sorghi) and Maize (Zea Mays L.) Sorghum downy mildew (SDM) caused by the oomycete, Peronosclerospora sorghi, is one of the destructive diseases that afflict maize. P. sorghi infects a susceptible host, hindering its growth and altering its morphology. To understand the molecular basis of the compatibility interaction between P. sorghi and maize, a comparative proteomic approach (2D-PAGE) was employed between the mock-inoculated (control) and SDM-inoculated leaves in the susceptible genotypes of maize (UMI79 and CM500). Seventeen spots showed a significant difference in the abundance of proteins in control and inoculated samples were further analyzed with MALDITOF/MS. The resulting peptide mass fingerprint was subjected to MASCOT analysis and it was found that most were related to stress that includes lipoxygenase and DEAD-box ATP-dependent RNA helicases, microtubule-associated protein and probable protein disulphide isomerase. Additionally, proteins involved in the cell cycle/endoreplication such as DNA topoisomerase 6 subunit A and retinoblastoma-related proteins were differentially expressed during infection. The possible roles of these proteins in response to P. sorghi infection in maize are discussed...