Abstract
Aging of the brain can result in excessive glucocorticoid secretion, potentially due to chronic stress and related situations. This can lead to dysfunction of brain areas involved in control of the hypothalamic-pituitary adrenal axis, growth, and metabolism, as well as areas associated with cognition and mood regulation. This chapter presents a protocol for two-dimensional differential in-gel electrophoresis (2D-DIGE) analysis of hypothalamus and hippocampus tissue obtained from mice following exposure to high levels of corticosterone for 14days. The chapter also presents a method for identification of the affected proteins in these brain regions using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry.
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