Proteomic Analysis of Anti-inflammatory Effects of a Kampo (Japanese Herbal) Medicine "Shoseiryuto (Xiao-Qing-Long-Tang)" on Airway Inflammation in a Mouse Model.

Takayuki Nagai,Haruki Yamada,Marino Nakao,Yuliko Shimizu,Tadakazu Maeda,Masamichi Oh-Ishi,Yoshio Kodera

doi:10.1093/ecam/nep151

Takayuki Nagai, Haruki Yamada + Show 5 more

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https://doi.org/10.1093/ecam/nep151

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Abstract

Effects of a Kampo (Japanese herbal) medicine “shoseiryuto (SST, xiao-qing-long-tang in Chinese)”, which has been used for the treatment of allergic bronchial asthma clinically, were examined on ovalbumin (OVA)-sensitized allergic airway inflammation model (i.e., bronchial asthma) in a mouse. When SST was orally administered at 0.5 g kg−1 day−1 from day 1 to 6 after OVA inhalation, SST reduced the inflammation in lung tissue, the number of eosinophils and the OVA-specific immunoglobulin E (IgE) antibody titer in bronchoalveolar lavage (BAL) fluids at 7 days after the OVA inhalation. SST also reduced the airway hyperreactivity at 6 days after the OVA inhalation. Proteomic analysis with the agarose two-dimensional electrophoresis showed that the expression of spectrin α2 was reduced in the lung tissue of OVA-sensitized mice and SST recovered the expression. Western blot and immunohistochemical analyses of lung tissue also confirmed this result. When prednisolone was orally administered at 3 mg kg−1 day−1 from day 1 to 6 after OVA inhalation, the inflammation in lung tissue, the number of eosinophils in BAL fluids and airway hyperreactivity were reduced in the OVA-sensitized mice. However, prednisolone did not reduce the OVA-specific IgE antibody titer in BAL fluids and did not recover the expression of spectrin α2 in lung tissue. These results suggest that at least a part of action mechanism of SST against OVA-sensitized allergic airway inflammation in a mouse model is different from that of prednisolone.

Highlights

Dramatic increases in the prevalence of bronchial asthma have occurred over the past few decades in Westernized countries and more recently in less-developed nations [1]
When SST was orally administered at 0.5 g kg−1 day−1 from day 1 to 6 after OVA inhalation, SST reduced the inflammation in lung tissue, the number of eosinophils and the OVA-specific immunoglobulin E (IgE) antibody titer in bronchoalveolar lavage (BAL) fluids at 7 days after the OVA inhalation
These results suggest that SST and prednisolone decrease the inflammation in the lung of the OVA-sensitized and OVA-challenged mice on histochemical analysis

Summary

Introduction

Dramatic increases in the prevalence of bronchial asthma have occurred over the past few decades in Westernized countries and more recently in less-developed nations [1]. Construction of airway smooth muscle and development of airway hyper-reactivity (AHR) are hallmarks of bronchial asthma [4,5,6]. In vitro antiallergic activities of SST include inhibition of histamine release and degranulation of mast cells [8, 9], proliferation of eosinophils [10], growth and differentiation of basophils [11], cholinergic effects for nasal gland acinar cells [12], synthesis of tumor necrosis factor α by peripheral blood mononuclear cells [13] and contraction of bronchial smooth muscle [14, 15]. We have reported that SST reduced the productions of Th2

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