Abstract
Proteomic analysis was used to generate a map of Populus deltoides CL. “2KEN8” mature pollen proteins. By applying 2-D electrophoresis, we resolved 403 protein spots from mature pollen. Using the matrix-assisted laser desorption/ionization time time-of-flight/time-of-flight tandem mass spectrometry method, we identified 178 distinct proteins from 218 protein spots expressed in mature pollen. Moreover, out of these, 28 proteins were identified as putative allergens. The expression patterns of these putative allergen genes indicate that several of these genes are highly expressed in pollen. In addition, the members of profilin allergen family were analyzed and their expression patterns were compared with their homologous genes in Arabidopsis and rice. Knowledge of these identified allergens has the potential to improve specific diagnosis and allergen immunotherapy treatment for patients with poplar pollen allergy.
Highlights
In spermatophytes, pollen grains are the dispersal agents of sperm cells and are vital for successful sexual reproduction and subsequent seed and fruit production (Sheoran et al, 2007)
Proteomic Maps of P. deltoides Mature Pollen After the 2-DE gels were aligned and matched, a total of 403 reproducible protein spots were detected in P. deltoides CL. “2KEN8” mature pollen
All detected protein spots were processed by automated ingel tryptic digestion and MALDI-TOF/TOF MS/MS analysis
Summary
Pollen grains are the dispersal agents of sperm cells and are vital for successful sexual reproduction and subsequent seed and fruit production (Sheoran et al, 2007). It has been reported that pollens are a major cause of Type I allergies due to the presence of several allergens (Nakamura and Teshima, 2013). More than 30% of the world population is affected by different kinds of allergy, caused by naturally occurring as well as synthetically produced compounds and their prevalence is increasing daily (Singh and Shahi, 2008). The proteome is the entire set of proteins expressed by a genome, cell, tissue, or organism (Wilkins et al, 1996). It is highly dynamic and depends on cell cycle, environmental influences and tissue/cell type. Rapid advances in proteomic technologies, along with completion of many
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