Abstract
BackgroundCitrus canker is a disease caused by Xantomonas citri subsp.citri (Xac), and has emerged as one of the major threats to the worldwide citrus crop because it affects all commercial citrus varieties, decreases the production and quality of the fruits and can spread rapidly in citrus growing areas. In this work, the first proteome of Xac was analyzed using two methodologies, two-dimensional liquid chromatography (2D LC) and tandem mass spectrometry (MS/MS).ResultsIn order to gain insight into the metabolism of Xac, cells were grown on two different media (NB - Nutrient Broth and TSE - Tryptone Sucrose broth enriched with glutamic acid), and proteins were proteolyzed with trypsin and examined by 2D LC-MS/MS. Approximately 39% of all predicted proteins by annotation of Xac were identified with their component peptides unambiguously assigned to tandem mass spectra. The proteins, about 1,100, were distributed in all annotated functional categories.ConclusionsThis is the first proteomic reference map for the most aggressive strain of Xanthomonas pathogen of all orange varieties. The compilation of metabolic pathways involved with bacterial growth showed that Xac expresses a complete central and intermediary metabolism, replication, transcription and translation machineries and regulation factors, distinct membrane transporters (ABC, MFS and pumps) and receptors (MCP, TonB dependent and metabolites acquisition), two-component systems (sensor and regulatory components) and response regulators. These data corroborate the growth curve in vitro and are the first reports indicating that many of these genome annotated genes are translated into operative in Xac. This proteomic analysis also provided information regarding the influence of culture medium on growth and protein expression of Xac.
Highlights
Citrus canker is a disease caused by Xantomonas citri subsp.citri (Xac), and has emerged as one of the major threats to the worldwide citrus crop because it affects all commercial citrus varieties, decreases the production and quality of the fruits and can spread rapidly in citrus growing areas
Proteomic analysis from Xac under two distinct conditions The Xanthomonas citri subsp. citri was grown at 28°C in Nutrient Broth (NB) or Tryptone Sucrose broth enriched with glutamic acid (TSE) media
The MS/MS spectra led to the identification of 1,162 proteins from bacteria grown in NB and 1,261 proteins from bacteria grown in TSE
Summary
Citrus canker is a disease caused by Xantomonas citri subsp.citri (Xac), and has emerged as one of the major threats to the worldwide citrus crop because it affects all commercial citrus varieties, decreases the production and quality of the fruits and can spread rapidly in citrus growing areas. About half of the predicted proteins have no inferable functions, especially in microorganisms [1,2]. Many approaches, such as two-hybrid systems and proteomics, have been used to describe genes that are effectively translated into proteins for systems of interest without considering transcriptomics or other methodologies that focus on RNA expression. They are Despite the large number of proteomic studies, for bacteria, there are few reports about the genus Xanthomonas. Most proteomic analyses involving organisms of the Xanthomonadaceae family have studied Xanthomonas campestris pv. campestris [4,5,6,7], due to the completion of its genome sequencing in 2002 [8] and its ability to infect Arabidopsis thaliana, a model plant for genetic studies [9]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
