Abstract
A feeding study was conducted to determine the effect of dietary nucleotides (NT) on growth performance and muscle proteome profile of rainbow trout fingerlings. Five experimental diets according to different levels of supplemented nucleotides (0, 0.05, 0.1, 0.15 and 0.2%) were assayed in rainbow trout for 8 weeks. Each diet was randomly allocated to triplicate groups of fish with initial average weight of approximately 23 g. The percentage of body weight gain (WG) and feed efficiency (FE) of fish were better when the fish were fed 0.15–0.2% diets. At the end of feeding trial, fish fed the basal and 0.2% diets were subjected to proteomic analysis. Proteins of the muscle tissue were analyzed using two-dimensional electrophoresis and MALDI-TOF/TOF mass spectrometry. Dietary NT caused differential expression of muscle metabolic proteins including glyceraldehyde-3-phosphate dehydrogenase, creatine kinase, adenylate kinase, nucleoside diphosphate kinase, and triosephosphate isomerase. In addition to metabolic enzymes, troponin-T-1 as a structural protein was found to increase in abundance in the treated fish. The altered expression of both metabolic and structural proteins in fish fed NT may be related to higher growth rate in rainbow trout. These findings provide basic information to understand possible mechanisms of dietary NT contribution to better growth in rainbow trout.
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