Abstract
ABSTRACTMaize genotypes P1-rr (R) and P1-ww (W) have high and low flavonoid content, respectively. Grain proteome profiles of these maize genotypes were compared. Two-dimensional gel electrophoresis (2-DE) was performed from three soluble protein extracts of each maize genotype and 55 proteins spots were differentially expressed using univariate analysis. Differentially expressed protein spots were analyzed by mass spectrometry-peptide mass fingerprint (MS-PMF) and eight of them were identified on the Zea mays L. database. Additionally, grouping of proteomic data was evaluated by principal component analysis (PCA) using 135 matched spots in all six analyzed gels. First component (PC1) represented 46.5% of total variation and second component (PC2) referred to 22.5%. This multivariate analysis showed the separation of the two maize genotypes proteome profiles using 2-DE data. The data presented in this study shows that 2-DE complemented with PCA is applicable to food analysis.
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