Abstract
The proteome of the marine bacterium Photobacterium angustum S14 was exposed to UVB and analyzed by the implementation of both the post-digest ICPL labeling method and 2D-DIGE technique using exponentially growing cells. A total of 40 and 23 proteins were quantified in all replicates using either the ICPL or 2D-DIGE methods, respectively. By combining both datasets from 8 biological replicates (4 biological replicates for each proteomics technique), 55 proteins were found to respond significantly to UVB radiation in P. angustum. A total of 8 UVB biomarkers of P. angustum were quantified in all replicates using both methods. Among them, the protein found to present the highest increase in abundance (almost a 3-fold change) was RecA, which is known to play a crucial role in the so-called recombinational repair process. We also observed a high number of antioxidants, transport proteins, metabolism-related proteins, transcription/translation regulators, chaperonins and proteases. We also discuss and compare the UVB response and global protein expression profiles obtained for two different marine bacteria with trophic lifestyles: the copiotroph P. angustum and oligotroph Sphingopyxis alaskensis.
Highlights
Research on the environmental effects of solar ultraviolet radiation in both terrestrial and aquatic ecosystems was largely stimulated by the discovery of an area of ozone depletion over Antarctica in the 1980’s, the so-called ‘ozone hole’
The key factors implicated in an adaptive response to solar radiation included DNA-binding proteins, proteins involved in the detoxification of toxic compounds, iron sequestration proteins that minimize oxidative stress, chaperones, proteins involved in nitrogen-related metabolism, and transcriptional/translational regulators [8]
The use of a combined proteomic analysis has allowed us to identify a set of biomarkers of the UVB stress response in P. angustum
Summary
Research on the environmental effects of solar ultraviolet radiation in both terrestrial and aquatic ecosystems was largely stimulated by the discovery of an area of ozone depletion over Antarctica in the 1980’s, the so-called ‘ozone hole’. The response of heterotrophic bacteria to UV radiation involves a highly organized series of intracellular events, enabling them to attenuate the damaging impact of UVB radiation. Only a few quantitative proteomics studies have explored the impact of damaging solar radiation on microorganisms. The key factors implicated in an adaptive response to solar radiation included DNA-binding proteins, proteins involved in the detoxification of toxic compounds (such as glyoxal and reactive oxygen species), iron sequestration proteins that minimize oxidative stress, chaperones, proteins involved in nitrogen-related metabolism, and transcriptional/translational regulators [8]. To determine the species-specific pathways involved in the UV response and fundamental biological processes commonly affected in microorganisms, there is still a need for more studies on damaging UV radiation using key model of marine bacteria
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