Proteome Analysis in a Mammalian Cell line Reveals that PLK2 is Involved in Avian Metapneumovirus Type C (aMPV/C)-Induced Apoptosis.

Rong Quan,Lei Hou,Jue Liu,Jing Wang,Moran Lv,Shanshan Zhu,Li Wei,Zixuan Li

doi:10.3390/v12040375

Abstract

Avian metapneumovirus subtype C (aMPV/C) causes an acute respiratory disease that has caused serious economic losses in the Chinese poultry industry. In the present study, we first explored the protein profile in aMPV/C-infected Vero cells using iTRAQ quantitative proteomics. A total of 921 of 7034 proteins were identified as significantly altered by aMPV/C infection. Three selected proteins were confirmed by Western blot analysis. Bioinformatics GO analysis revealed multiple signaling pathways involving cell cycle, endocytosis, and PI3K-Akt, mTOR, MAPK and p53 signaling pathways, which might participate in viral infection. In this analysis, we found that PLK2 expression was upregulated by aMPV/C infection and investigated whether it contributed to aMPV/C-mediated cellular dysfunction. Suppressing PLK2 attenuated aMPV/C-induced reactive oxygen species (ROS) production and p53-dependent apoptosis and reduced virus release. These results in a mammalian cell line suggest that high PLK2 expression correlates with aMPV/C-induced apoptosis and viral replication, providing new insight into the potential avian host cellular response to aMPV/C infection and antiviral targets.

Highlights

Avian metapneumovirus is the causative agent of respiratory tract infections and reduced egg production in poultry and has caused global economic losses [1,2]. aMPV was first reported inSouth Africa in 1978 [3] and was subsequently reported in many countries worldwide
Typical cytopathic effects (CPEs) were observed in Vero cells infected with Avian metapneumovirus subtype C (aMPV/C) JC
Western blot analysis of the aMPV/C JC infection showed that F protein was already detectable at 12 hpi (Figure 1B)

Summary

Introduction

South Africa in 1978 [3] and was subsequently reported in many countries worldwide. Both aMPV and human metapneumovirus (hMPV) belong to the new Pneumoviridae family, genus Metapneumovirus. We isolated a subtype C aMPV (aMPV/C), designed strain JC, from the infected chickens and showed that it was the causative agent. This was the first report of chickens infected with aMPV/C [11].

Methods

Results

Conclusion