Abstract
A procedure for surveying mold strains for proteinase production under submerged culture conditions is described. Four hundred and ninety-one strains of the Aspergillus flavus-oryzae group were tested. Eighty strains produced sufficient proteinase activity to warrant further study. On an over-all basis, greatest activity was found at pH 7.5, much less at pH 5.0, and no appreciable activity at pH 2.5. Ability to hydrolyze gelatin occurred frequently, and milk-clotting activity was rare. High α-amylase activity occurred infrequently and was not associated with high proteinase activity. Individual mold strains varied in their ability to attack various substrates. Seventeen strains showing superior proteinase or α-amylase activity are listed with quantitative data regarding their enzyme activities.
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