Proteolytic Activity in Meadow Soil after the Application of Phytohormones.

Holik Holik,Vranová Vranová

doi:10.3390/biom9090507

Abstract

Phytohormones, similar to soil enzymes, are synthesized and secreted into the soil environment by fungi and microorganisms. Phytohormones are involved in regulating microbial community activity in the rhizosphere. This paper examines how auxins, cytokinins, ethephon and chlorocholine chloride affect the activity of native soil proteases in the organo-mineral horizon of an alpine meadow. In the meadow habitat, native soil proteases were inhibited by auxins whereas the effect of cytokinins on these enzymes was not statistically significant. A similar inhibitory effect on the activity of proteases was shown for ethephon and chlorocholine chloride, both of which also inhibited the activity of native soil proteases in the alpine meadow soil. Overall, the inhibitory effect of phytohormones on the activity of native protease activity may affect plant nutrition by retarding the nitrogen cycle in the soil. This work contributes to our understanding of the influence of substances produced by the rhizosphere that can actively participate in the activity of soil microorganisms and consequently influence the soil nitrogen cycle.

Highlights

One of the most important natural resources is the soil, on which plants, animals and micro-organisms are directly or indirectly dependent [1,2]
Significant inhibition of native proteases occurred in response to the indole-3-acetic acid (IAA) and naphthoxyacetic acid (NOA) auxins, both of which led to a large activity reduction at 50 and 100 μg (IAA and NOA) (Table 2)
The smallest inhibition of the native protease activity was achieved with the synthetic auxin naphthaleneacetic acid (NAA) and only at 50 μg NAA

Summary

Introduction

One of the most important natural resources is the soil, on which plants, animals and micro-organisms are directly or indirectly dependent [1,2]. The decomposition of organic matter produced by plants, animals and microorganisms is an important ecological process that ensures bioavailable forms of nutrients bound to it, and is mediated in the soil by extracellular enzymes. These enzymes are produced by plants and microorganisms [3]. A diverse array of organisms in the soil play a key role in pedogenesis and nutrient cycling through various interactions [1,2]. Microorganisms interact with plants, directly affecting their growth [6]

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Results

Conclusion