Abstract
Resistant and sensitive strains of mice (BSVS and BRVR) and rats (Lewis and Brown Norway) were used as models for determining if changes in proteolytic enzymes occur as a result of experimental allergic encephalomyelitis (EAE). The discrete anatomical areas affected by EAE were analyzed for acid proteinase (with hemoglobin as substrate), cathepsin A (carbobenzoxy (Z)-Glu-Tyr), aminopeptidase (Leu-Gly-Gly) and arylamidase (Arg-βNA). (1) In the sensitive Lewis rats, EAE was accompanied by a significant rise in acid proteinase (E.C. 3.4.4.23) in spinal cord homogenates from 8.4 reaching a maximum of 13.1 μmoles/g fresh weight, 20 days after sensitization. No comparable changes were seen in cathepsin A activity during the same period. Activity of these two enzymes in spleen was unaffected by EAE. (2) In BSVS (sensitive) and BRVR (resistant) strains of mice no significant changes occurred in the ventricular angles, or the perivenous region of the upper vermis of the cerebellum for any of the proteolytic enzymes. Sensitization in both strains was accompanied by increased organ weights of spleen and thymus but with a more rapid return to control values in the resistant BRVR strain. No enzymatic changes in liver homogenates were observed in both strains. The significance of these changes in relation to susceptibility to EAE in different strains and species is discussed.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
