Abstract
[3H]-labelled Casein and chromogenic substrates were used for a quantitative determination of protease activity in cell culture supernatants during long-term cultivation of BHK and hybridoma cells. They produced recombinant human interleukin 2 and an IgG2a - antibody respectively in serum-or protein free medium. Characterization of protease activities was performed by inhibitor studies and specific p-NA derivates. Only 20% of the total protease activity in hybridoma and up to 50% in BHK cell supernatants is based on serine type proteases. Supplementation of supernatants with 2-mercaptoethanol or magnesiumchloride caused an increase of protease activities.
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