Abstract

AbstractLucerne (Medicago sativa), field wilted to 330 g dry matter (DM) kg−1, and whole‐plant maize (Zea mays), 383 g DM kg−1, were treated with formic acid (FA) at the rate of 4‐5 1 t−1 fresh forage. The effects of FA treatment on proteolysis and amino acid degradation was investigated at several intervals over a 90‐d period of ensilage in laboratory silos. Formic acid treatment produced different patterns of pH decline during ensilage of the two forages, suggesting some degree of crop specificity in response to FA treatment. After 7 d of ensilage of lucerne there were increases of 81% in nonprotein nitrogen (NPN), and 104% in free amino acid nitrogen (FAA‐N), but with FA‐treated lucerne the increases were 39% and 31%, respectively. FA treatment resulted in a reduction in the concentration of most free amino acids in lucerne silage, with the exception of glutamic acid and serine; the branched chain amino acids as well as glycine, tyrosine and proline were significantly (P<0·05) reduced after 3 d of ensilage. By contrast, FA treatment of maize did not significantly (p<0·05) affect the NPN content nor was there a consistent pattern in the changes in FAA‐N. Formic acid treatment of maize did produce a significant reduction (P<0·05) in the concentration of most individual free amino acids at 90 d of ensilage. Overall, FA treatment had a more predictable effect on proteolysis and amino acid degradation in ensiled lucerne than in ensiled whole‐plant maize.

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