Proteoglycans from squid cranial cartilage: extraction and characterization

Abstract

Abstract 1. 1. The majority of proteoglycans (85% of uronic acid) were extracted from squid cranial cartilage with 2% SDS. 2. 2. Conventional methods with 4 M Gdn HCl alone or with 4% Zwittergent, extracted only 30%. 3. 3. The proteoglycans were isolated by density gradient centrifugation. 4. 4. At least 25% of the extracted proteoglycans with 2% SDS do not form aggregates with hyaluronate. 5. 5. The proteoglycan monomers are of varying hydrodynamic size, those extracted with 4 M Gdn HCl being the smallest of average molecular weight 750,000.