Proteins sequence analysis of Contagious Caprine Pleuropneumonia

Ayuba Dauda,Abdulmojeed Yakubu,Ihe Dim,Deeve Gwaza

doi:10.2298/bah1703309y

Abstract

A total of twenty (20) contagious bovine pleuropneumonia (CCPP) proteins were retrieved from the GenBank (www.ncbi.nlm.nih.gov). The proteins sequences were used to investigate the molecular identity of various CCPP proteins. The physico-chemical properties of CCPP proteins were performed using protparam tool. Isoelectric point (pI), molecular weight (MW), extinction coefficient (EC); instability index (II), aliphatic index (AI) and grand average of hydropathicity (GRAVY) were computed. The study revealed that the pI of CCPP proteins were acidic and basic in nature. The EC and II of CCPP proteins indicate better stability which is an indication of resistant to mutation and thermally stable. The GRAVY of CCPP proteins revealed some are positive while some are negative. The positive value indicates solubility (hydrophilic) in water while negative is not soluble (hydrophobic) in water. The amino acid composition of CCPP proteins indicates that they are rich in isoleucine, leucine and lysine. The three dimensional structures (3D) of the CCPP proteins were determine using Phyre2 server. The amino acid sequences of CCPP proteins were subjected to secondary structure prediction using ExPASy?s SOPMA tool. The proteins are more of alpha helix structure. The genetic information eminating from this study may bring insight into mutagenesis and pharmacogenetic. This article has been retracted. Link to the retraction <a href="http://dx.doi.org/10.2298/BAH1803369E">10.2298/BAH1803369E</a>

Highlights

Contagious Caprine Pleuropneumonia (CCPP) is a devastating disease of goats included in the list of notifiable diseases of the Organization for Animal Health (OIE).The first description of the disease dates back to 1873, in Algeria (Thomas, 1873).CCPP is a contagious disease of goats, which occurs in per acute, acute or chronic forms and is characterized by fibrinous pneumonia, pleurisy and profuse pleural exudates (Edelsten et al, 1990)
The most powerful and accurate methods for detecting and aligning remotely related sequences rely on profiles or Hidden Markov Models (HMMs). 3DligandSite was used to predict the binding site of the 3D structure of the CCPP proteins
The computed isoelectric point values of CCPP proteins in the study revealed Phosphoglycerate kinase, Glycyl-tRNA Synthetase, ATPdependent protease La, GTP-Binding protein, tRNA Modification GTpass, LysinetRNA ligase and Chromosome segregation ATPase are acidic which have while the rest appeared to be basic in nature with

Summary

Introduction

Contagious Caprine Pleuropneumonia (CCPP) is a devastating disease of goats included in the list of notifiable diseases of the Organization for Animal Health (OIE).The first description of the disease dates back to 1873, in Algeria (Thomas, 1873).CCPP is a contagious disease of goats, which occurs in per acute, acute or chronic forms and is characterized by fibrinous pneumonia, pleurisy and profuse pleural exudates (Edelsten et al, 1990). Mortality rates of 60–100% are common (Edelsten et al, 1990). The disease is reported to occur in many countries in West and Eastern Africa and in Pakistan and India (OIE, 2001). The infectious agent Mycoplasma capricoleum subspecies capripneumoniae, formerly known as the F38-like group, is difficult to isolate and has only been identified in a few of the countries where the disease has been reported (Bolske et al, 1995a)

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Conclusion