Abstract
Lygeum spartum L. represent a natural barrier in the Algerian high plateaus against the advances of sand and turning into a desert. This species has two levels of ploA¯dy (diploA¯d cytotype 2n = 16 and polyploidy cytotype 2n = 40). Protein profile of several populations of this species have inter and intra specific variability. The polymorphic bands observed in the intermediate and slow zone have a molecular weight (MW) of 25.6, 30.1, 31.2, 37.3, 40.4, 69.3 and 82.7 kDa. This polymorphism exists not only between the polyploids samples (well 1 to 7) and diploids (well 8 and 9), but also inside the polyploids populations. These results showed that modifications of the electrophoretic profile of Lygeum spartum were concomitant with climatic and geographical external factors.
Highlights
Lygeum spartum L. is a typical perennial grass (Poaceae) of the Mediterranean basin. It occupies a significant surface in the Algerian high plateaus approximately 30.000 Km2[1]
Lygeum spartum is regarded as a natural barrier slowing down wind erosion and the stranding dunaire[2] thanks to its very developed roots system
There is a linear relation between the logarithm of Molecular weight molecular weight (MW) and the relative mobility of the bands, this allowed us to determine the MW of proteins starting from a calibration curve which was traced using the proteins markers of a known molecular mass: Phosphorylase B 97 kDa, Ovalbumin 45 kDa, Carbonic anhydrase 30 kDa, Trypsin inhibitor 20.1 kDa, provided by Amersham Pharmacia Biotech
Summary
Lygeum spartum L. is a typical perennial grass (Poaceae) of the Mediterranean basin. It occupies a significant surface in the Algerian high plateaus approximately 30.000 Km2[1]. Lygeum spartum is regarded as a natural barrier slowing down wind erosion and the stranding dunaire[2] thanks to its very developed roots system This species constitutes a good example of adaptation to the dryness[3], and to the salinity, its presence on salted grounds results from an adaptation of its roots system and more of the unfolding or tripling of the endoderm[4]. Tests of obtaining paper pulp starting from foliar tissues made it possible to confirm that the Lygeum spartum is a paper plant[7, 8]. This species is a plant of an ecological and economical interest. Sci., 3 (1): 337-341, 2008 from different areas in order to select interesting proteins and thereafter genes of the resistance to the dryness and salinity in these species for biotechnological applications
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