Abstract
Previous reports from our laboratory have shown that “crude” AG-e, a type-commons HSV antigen, induces an antigen-driven cell-mediated immune response in patients with HSV cervicitis or cervix cancer. Anti-crude AG-e sera give rise in crossed immunoelectrophoresis against total viral soluble antigenic mixtures (HSV-2 (G) SAM) to one precipitin band (“pure” AG-e). Pure AG-e immunologically identical to crude AG-e as determined by immunodiffusion and it resolves into two infected cell proteins, ICP 12 (MW: 140,000) and ICP 14 (MW: 130,000) upon SDS-acrylamide gel electrophoresis. ICP 12 and ICP 14 are purified to radiochemical homogeneity by SDS-acrylamide gel electrophoresis. Antisera to ICP 12 react with HSV-2 (G) SAM in crossed immunoelectrophoresis and both anti-ICP 12 and ICP 14 sera stain HSV-2 (G) infected cells. ICP 12and ICP 14 appear to be envelope proteins as evidenced by: (i) the absorption of the reactivity of anti-ICP 12 and ICP 14 sera with HSV-2 (G) virions but not with “mock-virus” preparations, (ii) the ability of the anti-ICP 12 and ICP 14 sera to neutralize HSV-2 in presence of anti-IgG, and (iii) the resolution of a protein with the relative mobility of ICP 14 in virions the surface of which was iodinated with lactoperoxidase.
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