Proteins of Friend leukemia cells. Comparison of hemoglobin-synthesizing and noninduced populations.

J L Peterson,E H Mcconkey

doi:10.1016/s0021-9258(17)33915-7

Abstract

Proteins of Friend leukemia cells induced to form large amounts of hemoglobin by dimethylsulfoxide treatment were compared with proteins from noninduced cells by high resolution two-dimensional polyacrylamide gel electrophoresis. Approximately 98% of more than 500 proteins separated by this technique were qualitatively and quantitatively the same in both cell populations. Changes representing more than 50% of the control cell amount were detected in six non-histone chromosomal proteins, two nucleoplasmic proteins, and three cytoplasmic proteins. It is concluded that dimethylsulfoxide induces an extremely specific pattern of erythroid differentiation in these cells, which should be susceptible to detailed analysis. Comparison of protein patterns from Friend leukemia cells and HeLa cells revealed electrophoretic identity of approximately 20% of cytoplasmic proteins and 50% of non-histone chromosomal proteins.

Highlights

Proteins of Friend leukemia cells induced to form large amounts of hemoglobin by dimethylsulfoxide treatment were compared with proteins from noninduced cells by high resolution two-dimensional polyacrylamide gel electrophoresis
We have used the high resolution two-dimensional polyacrylamide gel electrophoresis technique of O’Farrell (5) to ask whether erythropoietic differentiation of Friend leukemia cells involves a striking shift in the pattern of protein synthesis
Synthesis of Hemoglobin-Friend leukemia cells become visibly erythroid after a 4-day exposure to Me,SO; pelleted cells are distinctly red

Summary

AND NONINDUCED

From the Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder. Proteins of Friend leukemia cells induced to form large amounts of hemoglobin by dimethylsulfoxide treatment were compared with proteins from noninduced cells by high resolution two-dimensional polyacrylamide gel electrophoresis. Comparison of protein patterns from Friend leukemia cells and HeLa cells revealed electrophoretic identity of approximately 20% of cytoplasmic proteins and 50% of non-histone chromosomal proteins. Friend leukemia cells are erythroblastic cells of murine origin that can be induced to produce hemoglobin by treatment with 2% dimethylsulfoxide (1). We have used the high resolution two-dimensional polyacrylamide gel electrophoresis technique of O’Farrell (5) to ask whether erythropoietic differentiation of Friend leukemia cells involves a striking shift in the pattern of protein synthesis. ’ The abbreviations used are: Me,SO, dimethylsulfoxide; NHC proteins, non-histone chromosomal proteins

PROCEDURES

RESULTS

DISCUSSION

HeLa cells