Abstract
Protein crystals and bundles of tubules (tb) are induced inside of intact cells of Saccharomyces carlsbergensis and S. cerevisiae by incubation in hypertonic media both at room temperature and at 0 °C within a few minutes or by preparation of protoplasts. The induction is not possible in cells of Kluyveromyces fragilis and Rhodosporidium diabovatum. Formation of these structures depends on osmolality of the hypertonic media not on the kind of substances used. In cells previously cultured in medium containing glucose (5 %) instead of lactate (2 %) or ethanol (1 %) these structures can be induced only in few cases. Crystals and tb disappear within 1—4 hours provided that osmotic shock is followed by growth of cells or protoplasts.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
