Abstract
The release of prorenin as well as renin from rat renal slices was confirmed by a rat prorenin-prosegment ELISA system and an assay system for determining the renin activity. A significant increase of the prorenin release was found by adding rat submandibular gland extract to the slice medium, indicating the existence of a prorenin-releasing stimulator (PRS) in the extract. The p I and molecular mass of PRS were 8.5–8.7 and 28–30 kDa, respectively. The PRS was completely inactivated by boiling or a proteinase treatment. Renin releasing stimulator, pro-; Cortical slice; Submandibular gland; ELISA, prorenin-prosegment; (Rat kidney)
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